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Image Search Results
Journal: Aging and Disease
Article Title: Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice
doi: 10.14336/AD.2022.0623
Figure Lengend Snippet: Th17 and senescent T cells in LN induced by PD. (A) FACs scattergrams showing gating strategy for CD4+ T cells in LN. (B and C) IL-17 + CD4T cells and (D+E) CD28 - CD57 + expression in CD4T cells (N=6 per group, Two-way ANOVA and Tukey post-hoc multiple comparison test used (* p<0.05, **p<0.001, ***p<0.0001).
Article Snippet: Antibodies used: anti-human CD45R0 PerCP-eFluor 710; clone UCHL1 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#46-0457-41), anti-human CD27 eFluor 450; clone 0323 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#48-0279-42), anti-human IgD FITC; clone IA6-2 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#11-9868-42), anti-human CD57 PE Cyanine7; clone TB01(ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#25-0577-42), anti-human CD14 eFluor 450; clone 61D3 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#48-0149-42), anti-human CD3 PerCP Vio700; clone BW264/56 (Miltenyi Biotech Auburn, CA, USA; Cat #130-097-582), anti-human CD3 FITC; clone BW264/56 (Miltenyi Biotech Auburn, CA, USA; Cat #130-080-401), anti-human CD45RA VioGreen (Miltenyi Biotech Auburn, CA, USA; Cat#130-098-184), anti-human CD4 VioBlue; clone VIT4 (Miltenyi Biotech Auburn, CA, USA#130-098-163), anti-human CD16 VioGreen (Miltenyi Biotech Auburn, CA, USA; Cat#130-098-095), anti-human CD28 FITC; clone
Techniques: Expressing, Comparison
Journal: Aging and Disease
Article Title: Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice
doi: 10.14336/AD.2022.0623
Figure Lengend Snippet: Intragingival injection of exosomes promotes Th-17 + , senescent CD4 + CD28 - CD57 + T cells and inflammatory profile in DCs in LN. Cervical lymph nodes of young mice subjected to LIPD and injected with PgDCexo +/_ Rap or ImDCexo (control group) were harvested, cells isolated, labeled and analyzed by FACS (A) Gating strategy for T cells in LN (B, C) %IL17a + CD4 T cells (N=6 per group) (D, E) % CD28-CD57 + senescent CD4 T cells (N=6 per group). (F-K) FACS Intracellular staining showing inflammatory cytokine profile of DCs in LN; (F, G) IL-1β, (H, I) TNFa, and (J, K) IL-6, cells gated on CD11c + MHCII + (gate not shown) (N=6 per group). One way ANOVA and Tukey post hoc multiple comparison test used (*p<0.05, **p<0.001, ***p<0.0001) ImDCexo = exo from un-infected immature DCs (control), PgDCexo= exo from DCs infected with Pg.
Article Snippet: Antibodies used: anti-human CD45R0 PerCP-eFluor 710; clone UCHL1 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#46-0457-41), anti-human CD27 eFluor 450; clone 0323 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#48-0279-42), anti-human IgD FITC; clone IA6-2 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#11-9868-42), anti-human CD57 PE Cyanine7; clone TB01(ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#25-0577-42), anti-human CD14 eFluor 450; clone 61D3 (ebioscience, Thermofisher Scientific, Waltham MA, USA; Cat#48-0149-42), anti-human CD3 PerCP Vio700; clone BW264/56 (Miltenyi Biotech Auburn, CA, USA; Cat #130-097-582), anti-human CD3 FITC; clone BW264/56 (Miltenyi Biotech Auburn, CA, USA; Cat #130-080-401), anti-human CD45RA VioGreen (Miltenyi Biotech Auburn, CA, USA; Cat#130-098-184), anti-human CD4 VioBlue; clone VIT4 (Miltenyi Biotech Auburn, CA, USA#130-098-163), anti-human CD16 VioGreen (Miltenyi Biotech Auburn, CA, USA; Cat#130-098-095), anti-human CD28 FITC; clone
Techniques: Injection, Control, Isolation, Labeling, Staining, Comparison, Infection